Abstract:
Coal mining caused surface subsidence of large areas, destroyed the original land topography, soil and vegetation, so that affected the composition of soil microbial community. In order to investigate the correlation between AMF diversity in the mining subsidence area and mining time series, we explored the changes of diversity and population structure of AMF and its correlation with soil physicochemical properties with mining subsidence time in the Bulianta mining area. Results showed that 12 AMF species belonged to 5 genera were found in the study area.
Glomus was the dominant population, and
Acaulospora was the common population. The AMF species was different among years, and the distribution of genera and species was differed among different sites. With the increase of mining subsidence time, AMF diversity did not show regular changes. However, there was a significant negative correlation (
P < 0.01) between mining age and soil water, pH value, alkali hydrolyzed nitrogen, and a significant positive correlation (
P < 0.01) with available potassium. A positive correlation (
P < 0.05) between AMF spore density and soil alkaline phosphatase activities, and a significant negative correlation (
P < 0.01) between species richness and soil water content were also observed. AMF diversity was significantly negatively correlated (
P < 0.05) with soil water content, and positively correlated (
P < 0.05) with available potassium and alkali hydrolyzed nitrogen. The mining time affected soil physicochemical properties, and further affected the AMF diversity. Along with the mining time series and continuous reclamation, soil fertility was improved, and species diversity of AMF was recovered.