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添加螯合剂诱导-栽培红叶菾菜(Beta vulgaris var. cicla L.)修复铅和镉污染土壤效果的研究

马叶 赵国梁 王晓凤 成杰民

马 叶, 赵国梁, 王晓凤, 成杰民. 添加螯合剂诱导-栽培红叶菾菜( Beta vulgaris var. cicla L.)修复铅和镉污染土壤效果的研究[J]. 土壤通报, 2021, 52(2): 416 − 424 doi: 10.19336/j.cnki.trtb.2020062801
引用本文: 马 叶, 赵国梁, 王晓凤, 成杰民. 添加螯合剂诱导-栽培红叶菾菜( Beta vulgaris var. cicla L.)修复铅和镉污染土壤效果的研究[J]. 土壤通报, 2021, 52(2): 416 − 424 doi: 10.19336/j.cnki.trtb.2020062801
MA Ye, ZHAO Guo-liang, WANG Xiao-feng, CHENG Jie-min. Remediation of Lead and Cadmium Contaminated Soil with Chelate-Induced- Beta Vulgaris Var. Cicla L doi: 10.19336/j.cnki.trtb.2020062801
Citation: MA Ye, ZHAO Guo-liang, WANG Xiao-feng, CHENG Jie-min. Remediation of Lead and Cadmium Contaminated Soil with Chelate-Induced- Beta Vulgaris Var. Cicla L doi: 10.19336/j.cnki.trtb.2020062801

添加螯合剂诱导-栽培红叶菾菜(Beta vulgaris var. cicla L.)修复铅和镉污染土壤效果的研究

doi: 10.19336/j.cnki.trtb.2020062801
基金项目: 国家重点研究开发计划(No.2018YFD0800106-04和No.2018YFF0213404)资助
详细信息
    作者简介:

    马叶:马 叶(1996−),女,山东济南人,硕士研究生,主要从事土壤污染修复研究。Email: maye1996668@163.com

    通讯作者:

    Email: jmcheng2002@hotmail.com

  • 中图分类号: S147.2

Remediation of Lead and Cadmium Contaminated Soil with Chelate-Induced-Beta Vulgaris Var. Cicla L.

  • 摘要: 以雄安新区安新县重金属污染农田土壤为供试土壤,以Cd超积累植物红叶菾菜(Beta vulgaris var. cicla L.)为供试植物,设置不同浓度EDTA和柠檬酸(0,2.5,5,7.5,10 mmol kg−1)处理进行盆栽试验,探究螯合诱导-红叶菾菜修复Cd、Pb污染土壤的可行性。结果表明:(1)与对照相比,添加EDTA螯合剂使红叶菾菜生长及生物量均受到抑制,一定浓度柠檬酸处理能显著促进植物生长,5 mmol kg−1柠檬酸处理对植物株高、茎粗及生物量与对照相比的上升比例分别为4.52%、44.07%和50%;(2)添加EDTA螯合剂后土壤中Cd、Pb有效态含量相比对照分别提高了108.61% ~ 235.39%、67.98% ~ 224.16%,柠檬酸处理后土壤Cd、Pb有效态含量最大提高了180.07%、186.01%,EDTA对土壤重金属的活化效率显著高于柠檬酸;(3)通过对红叶菾菜地上部Cd、Pb含量及富集系数比较发现,EDTA更能促进红叶菾菜对Pb的吸收,柠檬酸更能促进红叶菾菜对Cd的吸收;(4)螯合剂处理后土壤中铵态氮、有效磷、速效钾含量显著增加。就本文试验条件、供试材料而言,螯合诱导-红叶菾菜修复铅镉复合污染土壤是可行的。
  • 图  1  添加螯合剂对植物株高的影响

    CK代表对照(不添加螯合剂),E代表添加EDTA,CA代表添加柠檬酸,数字代表添加浓度(mmol kg−1),a代表CA处理组,b代表EDTA处理组,下同

    Figure  1.  Effects of different concentrations of chelating agents on plant height

    图  2  添加螯合剂对植物茎粗的影响

    Figure  2.  Effects of different concentrations of chelating agents on plant stem diameter

    图  3  添加螯合剂对土壤中有效态Cd含量的影响

    Figure  3.  Effects of different concentrations of chelating agents on the concentration of soil available Cd

    图  4  添加螯合剂对土壤中有效态Pb含量的影响

    Figure  4.  Effects of different concentrations of chelating agents on the concentration of soil available Pb

    图  5  施加螯合剂对红叶菾菜地上部吸收Cd、Pb的影响

    Figure  5.  Effects of chelating agents on Cd and Pb concentrations of aboveground plants

    图  6  施加螯合剂对植物重金属富集系数的影响

    Figure  6.  Effects of chelating agents on enrichment coefficients of heavy metal in plants

    图  7  施加螯合剂对土壤pH的影响

    Figure  7.  Effects of chelating agents on soil pH values

    表  1  供试土壤基本理化性质

    Table  1.   Physical and chemical properties of soil

    pH < 0.002 mm粘粒
    Percentage of <
    0.002 mm clay(%)
    有机质
    Organic matter
    (g·kg−1
    铵态氮
    Ammonium nitrogen
    (mg·kg−1
    有效磷
    Available phosphorus
    (mg·kg−1
    速效钾
    Available potassium
    (mg·kg−1
    重金属(mg·kg−1
    Pollutant content
    总铅
    Total lead
    总镉
    Total cadmium
    有效态铅
    Available lead
    有效态镉
    Available cadmium
    8.55 30.33 14.74 17.19 33.04 131.78 462.00 3.83 102.00 0.88
    下载: 导出CSV

    表  2  试验处理

    Table  2.   Treatments of the experiment

    处理
    Treatment
    螯合剂
    Chelant
    浓度(mmol kg−1
    Concentration
    用量(g 盆−1
    Dosage
    CK 0 0
    CA2.5 CA 2.5 0.788
    CA5.0 5.0 1.575
    CA7.5 7.5 2.363
    CA10.0 10.0 3.150
    E2.5 EDTA 2.5 1.395
    E5.0 5.0 2.790
    E7.5 7.5 4.185
    E10.0 10.0 5.580
    下载: 导出CSV

    表  3  添加螯合剂对植物生物量的影响

    Table  3.   Effects of different concentrations of chelating agents on plant biomass

    处理
    Treatment
    鲜重(g 盆−1
    Fresh weight
    干重(g 盆−1
    Dry weight
    地上部
    Aboveground
    地下部
    Underground
    地上部
    Aboveground
    地下部
    Underground
    CK 12.25 ± 1.01 a 0.91 ± 0.10 a 1.16 ± 0.05 a 0.08 ± 0.01 a
    E2.5 7.66 ± 0.98 b 0.37 ± 0.02 b 0.82 ± 0.04 b 0.04 ± 0.01 b
    E5.0 6.72 ± 0.48 bc 0.29 ± 0.02 bc 0.68 ± 0.07 c 0.03 ± 0.01 bc
    E7.5 5.76 ± 0.42 c 0.20 ± 0.02 cd 0.57 ± 0.06 d 0.02 ± 0.01 bc
    E10.0 3.10 ± 0.21 d 0.18 ± 0.02 d 0.31 ± 0.02 e 0.01 ± 0.01 d
    CK 12.25 ± 1.01 cd 0.91 ± 0.10 a 1.16 ± 0.05 d 0.08 ± 0.01 c
    CA2.5 14.96 ± 0.93 ab 0.93 ± 0.22 a 1.58 ± 0.08 b 0.11 ± 0.01 ab
    CA5.0 15.91 ± 0.63 a 1.08 ± 0.05 a 1.71 ± 0.02 a 0.13 ± 0.01 a
    CA7.5 13.46 ± 0.76 bc 0.92 ± 0.04 a 1.39 ± 0.04 c 0.10 ± 0.01 bc
    CA10.0 10.79 ± 0.76 d 0.85 ± 0.04 a 1.16 ± 0.08 d 0.09 ± 0.01 bc
      注:均为平均值 ± 标准差,不同字母表示在0.05水平上差异显著,下同。
    下载: 导出CSV

    表  4  土壤有效态Cd、Pb含量与红叶菾菜植株体Cd、Pb含量的相关系数

    Table  4.   The correlation coefficients between available Cd and Pb in soil and Cd and Pb in plants

    处理
    Treatment
    植物地上部重金属浓度
    Concentration of heavy metal in plant aboveground
    DTPA-CdDTPA-Pb
    EDTA处理 0.953* 0.722
    CA处理 0.671 0.770
      注:*表示在0.05水平(双侧)上显著相关。
    下载: 导出CSV

    表  5  施用螯合剂对土壤养分形态的影响

    Table  5.   Effects of different chelating agents on soil fertility

    处理
    Treatment
    铵态氮
    Ammonium nitrogen
    有效磷
    Available phosphorus
    速效钾
    Available potassium
    含量(mg kg−1
    Content
    上升百分比(%)
    Percentage increase
    含量(mg kg−1
    Content
    上升百分比(%)
    Percentage increase
    含量(mg kg−1
    Content
    上升百分比(%)
    Percentage increase
    CK 17.73 ± 0.49 a 3.16 34.30 ± 0.04 a 3.82 136.78 ± 3.58 a 3.79
    E2.5 22.64 ± 0.19 b 31.75 44.59 ± 0.42 b 34.97 157.53 ± 8.20 b 19.54
    E5.0 24.75 ± 0.09 bc 43.99 47.34 ± 0.16 bc 43.29 163.32 ± 3.80 b 23.93
    E7.5 25.06 ± 0.16 c 45.83 48.39 ± 0.33 bc 46.46 183.40 ± 2.34 bc 39.17
    E10 26.84 ± 0.18 c 56.20 49.97 ± 0.56 c 51.24 199.04 ± 7.92 c 51.04
    CK 17.73 ± 0.49 a 3.16 34.30 ± 0.04 a 3.82 136.78 ± 3.58 a 3.79
    CA2.5 21.07 ± 0.21 b 22.57 36.17 ± 0.10 ab 9.48 142.65 ± 5.81 ab 8.25
    CA5.0 22.97 ± 0.04 b 33.63 39.13 ± 0.21 b 18.45 161.84 ± 2.20 b 22.81
    CA7.5 23.84 ± 0.02 c 38.74 44.30 ± 0.41 bc 34.08 175.21 ± 5.80 c 32.95
    CA10 24.45 ± 0.01 c 42.24 45.30 ± 0.25 c 37.11 181.45 ± 6.34 c 37.68
    下载: 导出CSV
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  • 收稿日期:  2020-06-28
  • 修回日期:  2020-08-07
  • 刊出日期:  2021-04-08

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