Abstract: In order to explore the temporal and spatial variation characteristics of soil nutrients and their correlations with topographical factors in tobacco-rice multiple-cropping area of eastern Hunan, the soil nutrients in Chaling in 2009, 2014 and 2018 were analyzed by using the traditional statistical and geostatistical analysis methods. The results showed that the contents of soil organic matter (SOM) and available potassium (AK) were at low levels, pH and the content of total nitrogen (TN) were at suitable level, and the content of total phosphorus (TP) was very low in 2009. From 2009 to 2014, soil pH value was decreased, while the contents of other nutrients were all increased. From 2014 to 2018, the pH value of soil was increased, the contents of SOM and TN were decreased, and the contents of TP, AK and water soluble chlorine were increased. The effects of random factors on soil pH and organic matter were more and more significant with the increase of years of tobacco rice multiple cropping. The contents of available potassium and water-soluble chloride always showed strong spatial correlation. Topographic factor analysis showed that the interaction between period and topographic factor was not significant. Different slopes had significant effects on soil pH and SOM content. Slope direction had strong effects on the distribution of available nutrients contents in soil. The cultivation system in the tobacco-rice multiple-cropping area of Chaling in the eastern Hunan was beneficial to improve soil fertility. Thus, increasing the application of N fertilizer, stable phosphate fertilizer input and reducing the application of K fertilizer could ensure the sustainable development of soil fertility.