Abstract: In order to determine the application effect of multi-layer coated controlled release mixed fertilizer in agricultural production and its effect on crop nutrient absorption, the field experiment was conducted to study its effect on garlic bulb, garlic bolt yield, nutrient absorption of different organs and soil available nutrient status. The results showed that under 10% reduction of total nutrient, the yield of garlic bolt and bulb increased by 32.74% and 10.61% respectively, compared with the local conventional fertilization, and 23.22% higher than that of single coated controlled release mixed fertilizer. Meanwhile, the application of multi-layer coated controlled release mixed fertilizer could improve the contents of soil available nitrogen. The contents of nitrate nitrogen and ammonium nitrogen in the harvest period were 23.79% and 5.79% higher than that of single coated controlled release mixed fertilizer. Under 30% reduction of total nutrient, the nitrogen content of bulb in multi-layer coated controlled release mixed fertilizer was 6.67% higher than that of single coated controlled release mixed fertilizer, which promoted the accumulation of phosphorus in bulbs and reduced the effect of nutrient accumulation to the upper part of the ground under nutrient stress. Through the analysis of economic benefits, it could be seen that under 10% reduction of total nutrient, the net income of the application of multi-layer coated controlled release mixed fertilizer was 6.83% higher than that of single coated controlled release mixed fertilizer, which had a broad application prospect in agricultural production.