Abstract: Winter pruned grape branches as raw materials, cake fertilizer, urea and ammonium bicarbonate as N sources, EM Fermentation Agent, biological bacterial fertilizer fermentation agent and straw fermentation agent as inoculating exogenous microorganisms. There were nine treatments for 60 days composting. The changes of compost temperature, physical properties of compost substrate, pH value, compost maturity and nutrient content under different control measures were studied. The results showed that adding different N sources to grape cuttings had a great influence on the heap temperature. Adding cake fertilizer increased the duration of high temperature above 50 ℃, The treatment 1 (adding cake fertilizer, C/N 20∶1, inoculating with EM Fermentation Agent) had the longest high temperature maintenance time, which was 10 days. At the end of composting, the bulk density, total porosity, aeration porosity and water holding porosity of each treatment were increased, and all were in the ideal matrix range. The pH value of each treatment was basically the same, which increased first and then decreased. At the end of composting, except for treatment 6 (adding ammonium bicarbonate, C/N 30∶1, inoculating microbial fertilizer starter), the germination index of other treatments was above 1, and the contents of N, P and K in treatment 1 (adding cake fertilizer, C/N 20∶1, inoculating with EM Fermentation Agent) were 3.160%, 0.959% and 1.880%, respectively, which had greater practical application value. Taken together, the best substrate fermentation ratio of grape cuttings was added cake fertilizer, adjusted C/N 20∶1, inoculated with EM Fermentation Agent.