溶磷菌Burkholderia ZP-4和Klebsiella ZP-2对土壤磷素的转化及细菌多样性的影响

Effect of Phosphate-Solubilizing Bacteria Burkholderia ZP-4 and Klebsiella ZP-2 on Soil Phosphorus Fraction and Bacterial Diversity

    摘要
  • 摘要:
      目的  探究溶磷菌接种对土壤磷组分及土壤细菌多样性和群落结构的影响，为亚热带地区土壤磷素形态的转化和利用提供科学依据。
      方法  向土壤中分别接种溶磷菌Burkholderia ZP-4和Klebsiella ZP-2菌液，进行室内培养试验，采用高通量测序技术检测土壤细菌OTUs丰度，分析溶磷菌处理后土壤特性和细菌群落多样性的变化。
      结果  溶磷菌显著降低了土壤pH值和有机质含量，使速效钾和速效氮含量显著增加。土壤H2O-Pi、NaHCO3-Pi、NaHCO3-Po、NaOH-Pi和NaOH-Po含量均增加，但HCl-Pi和Residual-P含量显著降低。与CK 相比，接种溶磷菌B. ZP-4和K. ZP-2处理后，土壤细菌的Simpson、Shannon、Chao1和ACE指数差异均不显著。在土壤细菌属分类学水平下，Olsenella、Fusobacterium、Streptococcus和Serratia等细菌的相对丰度显著增加。
      结论  添加溶磷菌ZP-4和ZP-2显著降低了土壤pH值，提高了土壤速效养分含量，加速了磷组分的转化，显著改变了土壤细菌的群落多样性。因此，溶磷菌B. ZP-4和K. ZP-2通过改变土壤pH，提高土壤速效养分含量，从而诱导土壤细菌改变了土壤磷组分，加速了磷素形态的转化。

     

    Abstract:
      Objective  To provide the conversion and utilization of soil phosphorus (P) and to form a certain scientific theory basis in subtropical areas, the effects of P-solubilizing bacteria (PSB) on P fractions and bacterial diversities and community structures in soil were studied.
      Method  Experiment was carried out when the suspension of Burkholderia ZP-4 and Klebsiella ZP-2 was inoculated. Soil bacterial OTUs was measured using the high-through sequencing, and then soil bacterial diversities and soil properties were analyzed at treatments of B. ZP-4 and K. ZP-2.
      Result  The soil pH value and soil organic matter content were decreased significantly at treatments of B. ZP-4 and K. ZP-2, but soil available potassium, available nitrogen concentrations had significant increased relative to control. Contents of H2O-Pi, NaHCO3-Pi, NaHCO3-Po, NaOH-Pi, NaOH-Po were increased but HCl-Pi and Residual-P concentrations were significantly decreased. Soil bacterial Simpson and Shannon diversity indices, Chao1and ACE richness estimators were all no significant differences. The relative abundances of Olsenella, Fusobacterium, Streptococcus, Serratia were significantly increased than that of control at genus level.
      Conclusion  The B. ZP-4 and K. ZP-2 were significantly decreased soil pH value, enhanced soil available nutrient, accelerated soil P element transformation, altered significantly soil bacterial community diversity. Hence, the B. ZP-4 and K. ZP-2 improved soil available nutrient contents and pH, inducted soil bacteria to alter soil P fraction, accelerate soil P cycling.

     

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