烤烟根际土壤化感物质因子分析及对烤烟经济性状的综合影响

Factor Analysis of Allelochemicals in Rhizosphere Soil of Flue-cured Tobacco and its Comprehensive Effect on Tobacco Economical Characteristics

  • 摘要:
      目的  通过分析烤烟根际土壤化感物质之间相互关系及与烤烟经济性状相关关系,为消减连作障碍配套研究提供理论参考。
      方法  以云南省红河州泸西连作烤烟为供试材料,利用GC-MS检测根际化感物质,通过因子分析和典型相关分析,提取能够解释大部分变量的公因子,分析根际化感物质与烤烟经济性状的相关关系。
      结果  烤烟根际检测并确定出13种根际土壤化感物质,因子分析提取3个公因子,第一因子为酚酸类物质,占有变量总方差的53.75%,对十四酸、莨菪亭、十七碳酸、亚麻酸具有高的正载荷,具有相似的变化趋势,相互之间呈正相关。第二因子为酸-酯-酰胺类因子,占变量总方差的19.27%,苯甲酸、香草醛、软脂酸、芥酸酰胺具有高的正载荷。第三因子为酚酯类因子,占变量总方差的11.78%,2,2′-亚甲基双-(6-叔丁基对甲苯酚)、邻苯二甲酸二辛酯具有高正载荷。简单相关和典型相关分析表明,根际化感物质不是单因子影响烤烟经济性状,是多因子综合影响,以第一因子为主要影响因素,具体表现为烤烟根际土壤十四酸、莨菪亭、十七碳酸和亚麻酸与单叶重和产值呈正相关,但与中上等烟叶比例呈负相关。
      结论  烤烟根际化感物质对烟叶的影响是双向的,但增加单叶重和亩产值的正效应大于降低中上等烟叶单叶重的负效应。

     

    Abstract:
      Objective  The study analyzed the correlation between the rhizosphere allelochemicals and tobacco economic characteristics and would provide a reference for decreasing the probolem caused by continuous cropping.
      Method  Continuous cropping tobacco from Yunnan Honghe Luxi were used as materials and allelochemicals were detected by GC-MS.
      Result  The results showed that 13 kinds of rhizosphere allelopathy substances were detected in rhizosphere soil of flue-cured tobacco. Three common factors were extracted by factor analysis. The first factor was phenolic acid factor, accounting for 53.752% of the total variance of variables. Myristic acid, belladonna pavilion, margaric acid and linolenic acid positively correlated with each other with high load. The second factor was acid-ester amide factor, accounting for 19.272% of the total variance of the variable. Benzoic acid, vanillin, palmitic acid, erucic acid amide had high positive loads. The third factor was phenolic ester factor, accounting for 11.778% of the total variance of variables. 2,2′-methylene bis -(6-tert-butylp-methylphenol) and dioctyl phthalate had high positive load. The results of simple correlation and canonical correlation showed that the effect of rhizosphere allelochemicals on tobacco economical characters was comprehensive and the first common factor was the main indicator. It behaved that myristic acid, belladonna pavilion, margaric acid and linolenic acid positively correlated with tobacco output value, while they negatively correlated with ration of middle-high grade leaves and weight per leaf class.
      Conclusion  Rhizosphere allelochemicals of tobacco had a bidirectional effect on tobacco. While the positive effects of increasing single leaf weight and output value were higher than decreasing ratio of middle-up class leaves. So it would provide a new thought and method for decreasing continuous cropping obstacle about how to make use of rhizosphere allelochemicals.

     

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