Objective  Soil microorganism is an important component of soil ecosystem and is highly sensitive to environmental changes. The composition, diversity and influencing factors of soil fungal community in Seabuckthorn plantation need to be explored in reclaimed land of mining area at different restoration years.

  Methods  The Seabuckthorn plantation with different restoration years in Juxinlong coal mine was taken as the research object and abandoned grassland as the control. The bacterial community in the collected soil samples was analyzed with high flux sequencing.

  Results  The results showed that there were differences in the composition of fungal community at different soil layers with different recovery years. There were 8 phyla, 30 classes, 69 orders, 164 families and 285 genera of fungi in the study area, and ascomycetes and basidiomycetes were the main flora, accounting for 98.2% of all phyla in the sample plot. With the increase of restoration years, the number of individuals and groups of the main groups at the taxonomic level of soil fungi showed an overall growth trend, which was higher than that of the grassland in the control sample plot. In addition, the richness and diversity of soil fungal communities in the sample plots restored for 7 years were significantly higher than those in other years and abandoned grasslands. Redundancy analysis showed that soil microbial community composition was mainly determined by soil pH and soil organic matter content, and was also related to soil nitrogen, phosphorus and potassium.

  Conclusion  The soil physical and chemical properties of Seabuckthorn plantation with different restoration years are different, and the increase of restoration years improves the species richness and diversity of fungi.