王 岳, 丁国栋, 刘梦婕, 高广磊, 于明含, 李 旭. 榆林沙区典型林地不同植被类型对土壤微生物群落结构的影响[J]. 土壤通报, 2022, 53(4): 907 − 918. DOI: 10.19336/j.cnki.trtb.2021120502
引用本文: 王 岳, 丁国栋, 刘梦婕, 高广磊, 于明含, 李 旭. 榆林沙区典型林地不同植被类型对土壤微生物群落结构的影响[J]. 土壤通报, 2022, 53(4): 907 − 918. DOI: 10.19336/j.cnki.trtb.2021120502
WANG Yue, DING Guo-dong, LIU Meng-jie, GAO Guang-lei, YU Ming-han, LI Xu. Influence of Different Vegetation Types on Soil Microbial Characteristics of Typical Forest Land in Yulin Sandy Area[J]. Chinese Journal of Soil Science, 2022, 53(4): 907 − 918. DOI: 10.19336/j.cnki.trtb.2021120502
Citation: WANG Yue, DING Guo-dong, LIU Meng-jie, GAO Guang-lei, YU Ming-han, LI Xu. Influence of Different Vegetation Types on Soil Microbial Characteristics of Typical Forest Land in Yulin Sandy Area[J]. Chinese Journal of Soil Science, 2022, 53(4): 907 − 918. DOI: 10.19336/j.cnki.trtb.2021120502

榆林沙区典型林地不同植被类型对土壤微生物群落结构的影响

Influence of Different Vegetation Types on Soil Microbial Characteristics of Typical Forest Land in Yulin Sandy Area

  • 摘要:
      目的  掌握榆林沙区典型林地土壤微生物特征,明确地上植被对土壤微生物群落结构的影响。
      方法  采集榆林沙区四种林分类型土壤,分析其土壤微生物群落结构。
      结果  测序共产生有效操作分类单元(OTU)15,509个,各林分间OTU及各类多样性指数没有显著性差异。优势菌种及其丰度土层间变化较大,但林分间优势细菌种一致,丰度排名前五的分别是变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)、厚壁菌门(Firmicutes)和绿弯菌门(Chloroflexi);樟子松、油松林下土壤中,酸杆菌门所占比例最大、分别达到22.32%和29.02%,而在沙柳和小叶锦鸡儿林下土壤中,变形菌门又成为优势菌,比例占到27.64%和28.51%。变形菌门和放线菌门在灌木林土壤中所占比例要高于乔木。
      结论  虽然各优势种丰度在林分间略有差别,但差异不显著(P < 0.05)。说明在一定区域内微生物群落结构复杂程度是受土壤本底的影响,不同季节或者土壤温度、湿度的变化对微生物群落结构的影响在一段时间后消除,群落结构归于稳定。

     

    Abstract:
      Objective  Soil microorganisms play a very important role in reflecting the influence of above-ground vegetation on soil, so the community structure of microorganism is needed to be clarified in soil.
      Method  the microbial community structures were analyzed in soils of 4 stands in Yulin Sand Area.
      Result  The results showed that Sequencing yielded a total of 15,509 effective operational taxonomic units (OTU) and apparent differences were not observed from the OTUs among stands or the diversity indicators of each unit. Dominant strain species presented large fluctuations in different soil layers, while converged among different stands. Their abundances varied considerably in different soil layers and the top-five dominant species included Proteobacteria, Actinobacteria, Acidobacteria, Firmicutes, and Chloroflexi. In the forest soils of P.sylvestris and P.tabulaeformis, Acidobacteria’s proportion maximized at 22.32% and 29.02% separately. In the forest soils of S.psammophila and C.microphylla, Proteobacteria became dominant and accounted for 27.64% and 28.51%, respectively. Proteobacteria and Actinobacteria owned higher proportions in shrub forest soils than that in arboreal forest soils.
      Conclusion  Despite of the slight abundance differences of dominant species among different stands, the differences were not significant (P < 0.05).The insignificant difference between forest stands showed that the microbial community structure in a certain area was more affected by the soil background. The influence of different seasons or changes in soil temperature and humidity on the microbial community structure will be eliminated after a period of time, and the community structure will be stable.

     

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