Abstract:
As an important component of total soil phosphorus (P), mineralized organic P (P
o) is a major source of replenishing bioavailable P pool in many terrestrial ecosystems. There are many forms of P
o with different bioavailability in soil. These forms of P
o also differ in their ability of resisting the hydrolysis catalyzed by phosphatases. It is therefore necessary to accurately determine the forms and mineralization rates of soil P
o to understand the biogeochemical cycling of P
o and to use the legacy P
o in soil. The aims were to clarify the advantages, disadvantages, scopes of application of several most commonly used approaches of determining the forms and mineralization rate of soil P
o. The selected 92 papers in the databases of CNKI and the Web of Science in recent 20 years with a topic of the forms and mineralization rate of soil P
o were discussed. Three approaches of determining the forms of soil P
o were reviewed, including sequential fractionation procedure, enzyme addition assays and
31P nuclear magnetic resonance spectroscopy. There is no a ‘best’ approach of determining the forms and mineralization rate of soil P
o. However, scientists can select a ‘proper’ one according to the physical-chemical properties of targeted soils and experimental goals. It is recommended to carry out more researches on the following fields: developing a standard procedure of enzyme addition assays, jointly utilizing
13C and
31P nuclear magnetic resonance spectroscopy to identify more unsolved P
o forms, modifying methods of determining mineralization rate of soil P
o to reflect the effects of plants, and combinedly utilizing approaches of determining the forms and mineralization rate to understand the mechanisms of transformation and mineralization of soil P
o.