杨 敏, 阳 珍, 胥 晓, 严贤春, 刘华峰, 陈亚梅. 不同植被恢复类型对四川泸石高速公路边坡土壤胞外酶及化学计量特征的影响[J]. 土壤通报, 2024, 55(1): 161 − 172. DOI: 10.19336/j.cnki.trtb.2022103002
引用本文: 杨 敏, 阳 珍, 胥 晓, 严贤春, 刘华峰, 陈亚梅. 不同植被恢复类型对四川泸石高速公路边坡土壤胞外酶及化学计量特征的影响[J]. 土壤通报, 2024, 55(1): 161 − 172. DOI: 10.19336/j.cnki.trtb.2022103002
YANG Min, YANG Zhen, XU Xiao, YAN Xian-chun, LIU Hua-feng, CHEN Ya-mei. Effects of Different Vegetation Restoration Types on Extracellular Enzymes and Stoichiometric Characteristics of Soil in Lu-shi Expressway Slopes, Sichuan Province[J]. Chinese Journal of Soil Science, 2024, 55(1): 161 − 172. DOI: 10.19336/j.cnki.trtb.2022103002
Citation: YANG Min, YANG Zhen, XU Xiao, YAN Xian-chun, LIU Hua-feng, CHEN Ya-mei. Effects of Different Vegetation Restoration Types on Extracellular Enzymes and Stoichiometric Characteristics of Soil in Lu-shi Expressway Slopes, Sichuan Province[J]. Chinese Journal of Soil Science, 2024, 55(1): 161 − 172. DOI: 10.19336/j.cnki.trtb.2022103002

不同植被恢复类型对四川泸石高速公路边坡土壤胞外酶及化学计量特征的影响

Effects of Different Vegetation Restoration Types on Extracellular Enzymes and Stoichiometric Characteristics of Soil in Lu-shi Expressway Slopes, Sichuan Province

  • 摘要:
      目的  研究不同植被恢复类型边坡土壤理化性质、胞外酶活性及其化学计量比,以及驱动土壤酶活性变化的主要因子,为边坡植被恢复提供科学依据和技术参考。
      方法  以泸石高速公路边坡裸地(BL)、恢复草地(RG)、恢复林地(RW)和自然草地(NG)为研究对象,测定土壤含水量、pH值、碳、氮、磷及酶活性等相关指标。
      结果  ①自然草地和恢复林地土壤含水量最高,裸地含水量最低,而自然草地和恢复草地土壤氮含量最高,裸地最低;②裸地土壤纤维二糖水解酶、β-葡萄糖苷酶、1,4-β-N-乙酰葡糖胺糖苷酶和酸性磷酸酶活性均低于其余三种植被类型;③除向量长度(VL)外,不同植被恢复类型土壤酶化学计量比差异显著,且向量角度均大于45°,这表明四种植被恢复类型土壤微生物更易受到磷元素限制;(4)冗余分析表明,土壤C/N、含水量、pH值、全C含量和N含量5个土壤因子共同解释土壤酶活性及其化学计量比变异的84.7%;其中,土壤C/N和含水量分别解释其变异的52.4%和39.2%。
      结论  不同植被恢复类型能显著影响土壤理化性质、胞外酶活性及其化学计量比,土壤C/N和含水量对土壤胞外酶活性及酶化学计量比起主要调控作用,土壤微生物生长和活性主要受磷元素限制。整体来看,自然草地和恢复草地要优于其他两种植被类型。

     

    Abstract:
      Objective  The physical and chemical properties, extracellular enzyme activities and stoichiometric ratio of soil in slope of different vegetation restoration types and the main factors driving the changes of soil enzyme activities were studied, in order to provide scientific basis and reference for slope vegetation restoration engineering.
      Method  Taking the bare land (BL), restored grassland (RG), restored woodland (RW) and natural grassland (NG) of Lu-shi expressway slope as the research objects, soil moisture content, pH, and C-, N- and P-acquiring enzymes in soil were measured.
      Result  ① The results showed that soil physical and chemical properties were significantly affected by vegetation restoration types. Soil moisture content was the highest in natural grassland and restored woodland and the lowest was in bare land, while soil nitrogen content was the highest in natural grassland and restored grassland and the lowest was in bare land. ②The activities of cellobiose hydrolase, β-glucosidase, 1, 4-β-N-acetylglucosaminidase and acid phosphatase in bare soil were lower than those of the other three vegetation types. ③ Except for vector length (VL), soil enzyme stoichiometric ratios of different vegetation restoration types were significantly different, and the vector angles were all greater than 45°, indicating that soil microorganisms of the four vegetation restoration types were more susceptible to phosphorus restriction.④ Redundancy analysis showed that five environmental factors, including soil C/N, moisture content, pH, total C content and soil N content, accounted for 84.7% of the variation of soil enzyme activities and stoichiometric ratios. Soil C/N and moisture content accounted for 52.4% and 39.2% of the variation, respectively.
      Conclusion  Different vegetation restoration types can significantly affect soil physicochemical properties, extracellular enzyme activities and its stoichiometric ratio. Soil C/N and water content have major regulatory effects on soil extracellular enzyme activities and enzyme stoichiometric ratio. In this study area, soil microbial growth and activity are mainly limited by phosphorus. On the whole, natural grassland and restored grassland were superior to the other two vegetation types.

     

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