湖南省油茶良种根际微生物区系分析

Analysis of Inter-root Microbiota of Improved Camellia oleifera Varieties in Hunan Province

  • 摘要:
    目的 为了弄清湖南省油茶主栽良种根际微生物区系特征,筛选出关键差异微生物菌群,为油茶林土壤生物菌肥研发提供参考。
    方法 利用高通量测序技术和OTU分析等方法,对湖南省油茶良种‘华硕’(HS)、‘华鑫’(HX)、‘湘林210’(XL210)以及普通油茶(CK)根际土壤微生物多样性及差异进行了分析,同时测定了油茶根际土壤理化性质及酶活性,运用冗余分析方法,探讨土壤因子与根际微生物之间的关系,并进行核心菌群的筛选。
    结果 ①油茶良种根际微生物数量的排序均为HS > HX > XL210 > CK。铁、铜、总有机碳、铵态氮、β-葡萄糖苷酶是影响土壤细菌多样性的主要因子,全钾、电导率、植酸酶是影响土壤真菌多样性的主要因子。②油茶良种与对照存在显著性差异的微生物为Microscillaceae、那提普斯壳科Natipusillaceae、Leotiomycetes。
    结论 差异微生物可作为人工合成菌群的核心候选成员,为油茶林土壤生物菌肥的研发奠定了基础。

     

    Abstract: :
    Objective The aims were to figure out the inter-root microbiota characteristics of Camellia oleifera varieties and screen out the key differential microflora in Hunan Province, which could provide an information for the development of soil biofertilizers in C. oleifera forests.
    Methods High throughput sequencing technology and OTU analysis were used to characterize the inter-root microbiota of C. oleifera varieties 'Huashuo' (HS), 'Huaxin' (HX), 'Xianglin 210' (XL210), and CK. The physicochemical and enzyme activity properties of C. oleifera inter-root soils were also determined. The relationship between soil factors and inter-root microorganisms was investigated by redundancy analysis (RDA) and screened out the core flora in C. oleifera.
    Results ① The order of the number of inter-root microorganisms in C. oleifera varieties was HS > HX > XL210 > CK. Fe, Cu, TOC, ammonium nitrogen, and β-glucosidase were the main factors affecting the diversity of soil bacteria. Total potassium, electrical conductivity, and phytase were the main factors affecting the diversity of soil fungi. ② Microorganisms with significant differences among different C. oleifera varieties were Microscillaceae, Natipusillaceae, unclassified_c_ Leotiomycetes.
    Conclusion These differential microorganisms could be used as the core candidate members, and laid an foundation for development of the soil biofungus fertilizer in C. oleifera forests.

     

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