基于原位酶谱技术镉污染土壤青葙根际酶活性空间分布特征研究

Spatial Distribution of Enzyme Activities in the Rhizosphere of Celosia argentea Linn. in Cd-contaminated Soil Using in-situ Soil Zymography

    摘要
  • 摘要:
    目的 为探明土壤镉污染对超富集植物青葙根际酶活性空间分布特征的影响。
    方法 基于土壤酶谱技术，对镉污染土壤和非污染土壤中青葙根际β-葡萄糖苷酶、酸性磷酸酶分布情况进行原位高分辨分析。
    结果 β-葡萄糖苷酶、酸性磷酸酶活性较高的热点区域都集中在青葙根系分布的区域，且在根尖处浓度较高。在污染土壤和非污染土壤中，青葙根部中心位置β-葡萄糖苷酶和酸性磷酸酶活性灰度值都显著高于距离根中心2 mm处酶活性灰度值。镉污染土壤青葙根部中心位置β-葡萄糖苷酶和酸性磷酸酶活性灰度值相较于非污染土壤，分别降低了5%和3%。
    结论 青葙根际土壤酶活性的空间分布具有显著的异质性，与根系分布密切相关，且土壤镉污染显著抑制了β-葡萄糖苷酶和酸性磷酸酶的活性。

     

    Abstract:
    Objective The aim was to investigate the influence of soil cadmium (Cd) contamination on the spatial distribution of β-glucosidase and acid phosphatase in the rhizosphere of Cd hyperaccumulator Celosia argentea Linn.
    Method The in-situ soil zymography was used to obtain the distribution of β-glucosidase and acid phosphatase in the rhizosphere of C. argentea in Cd-polluted soil and non-polluted soil.
    Result The Results showed that the hot spots of β-glucosidase and acid phosphatase were along entire root of C. argentea, which were relatively higher at the root tips. In both the polluted and non-polluted soils, the grey value of β-glucosidase and acid phosphatase at the root center were significantly higher than those 2 mm away from the root center. In Cd-polluted soil, the grey value of β-glucosidase and acid phosphatase in the root center decreased by 5% and 3% when compared to that in the non-polluted soil.
    Conclusion The results showed that the spatial distribution of soil enzyme activities was closely related to the root distribution. Soil Cd contamination had a significant negative influence on the soil enzyme activities.

     

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