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不同污染条件下毒死蜱在蚯蚓体内的蓄积特征及其生长影响

杨广斌 周世萍 熊张平 陈修才 朱鑫泽

杨广斌, 周世萍, 熊张平, 陈修才, 朱鑫泽. 不同污染条件下毒死蜱在蚯蚓体内的蓄积特征及其生长影响[J]. 土壤通报, 2021, 52(4): 920 − 926 doi: 10.19336/j.cnki.trtb.2020071401
引用本文: 杨广斌, 周世萍, 熊张平, 陈修才, 朱鑫泽. 不同污染条件下毒死蜱在蚯蚓体内的蓄积特征及其生长影响[J]. 土壤通报, 2021, 52(4): 920 − 926 doi: 10.19336/j.cnki.trtb.2020071401
YANG Guang-bin, ZHOU Shi-ping, XIONG Zhang-ping, CHEN Xiu-cai, ZHU Xing-ze. Bioaccumulation of Chlorpyrifos in Earthworm and Its Growth under Different Soil Pollution Conditions[J]. Chinese Journal of Soil Science, 2021, 52(4): 920 − 926 doi: 10.19336/j.cnki.trtb.2020071401
Citation: YANG Guang-bin, ZHOU Shi-ping, XIONG Zhang-ping, CHEN Xiu-cai, ZHU Xing-ze. Bioaccumulation of Chlorpyrifos in Earthworm and Its Growth under Different Soil Pollution Conditions[J]. Chinese Journal of Soil Science, 2021, 52(4): 920 − 926 doi: 10.19336/j.cnki.trtb.2020071401

不同污染条件下毒死蜱在蚯蚓体内的蓄积特征及其生长影响

doi: 10.19336/j.cnki.trtb.2020071401
基金项目: 国家自然科学基金项目(31860155)、云南省农业联合面上项目2017FG001(-040)和西南地区林业生物质资源高效利用国家林业和草原局重点实验室开放基金(2019-KF13)资助
详细信息
    作者简介:

    杨广斌(1995−),男,内蒙古呼伦贝尔,硕士研究生,主要从事土壤生态毒理学研究。E-mail: ygb849125904@163.com

    通讯作者:

    E-mail: kmzhoushiping@163.com

  • 中图分类号: X82

Bioaccumulation of Chlorpyrifos in Earthworm and Its Growth under Different Soil Pollution Conditions

  • 摘要: 以皮质远盲蚓(Amynthas corticis)为试验生物,采用一次、叠加的污染方式,研究了毒死蜱在土壤中的残留、蚯蚓体内的累积特征及对蚯蚓生长的影响。结果显示:相同实验浓度条件下,以叠加污染方式进入土壤的毒死蜱残留量低于一次污染方式的残留量。当土壤毒死蜱浓度为12 ~ 20 mg kg−1,暴露55 d时,一次污染条件下,蚯蚓体内毒死蜱蓄积量为3.73 ~ 8.84 mg kg−1,生物-土壤蓄积因子(FBSA)为1.55 ~ 1.70;叠加污染条件下,蚯蚓毒死蜱蓄积量为5.21 ~ 11.04 mg kg−1,FBSA为2.22 ~ 2.46。相同实验浓度条件下,叠加污染方式进入土壤的毒死蜱对蚯蚓的生长抑制显著高于一次污染处理组。该研究结果可为土壤毒死蜱生物毒性和生态风险评价提供基础数据。
  • 图  1  不同污染方式进入土壤的毒死蜱残留动态

    Figure  1.  Degradation dynamics of chlorpyrifos in soil after different treatments

    表  1  蚯蚓对不同污染方式进入土壤的毒死蜱蓄积

    Table  1.   Effect of different chlorpyrifos application on chlorpyrifos accumulation in earthworms

    处理组(mg·kg−1
    Treatment
    蚯蚓体内毒死蜱蓄积量(mg·kg−1
    The chlorpyrifos accumulation in earthworms
    21 d28 d35 d45 d55 d
    4(一次污染) 1.01 ± 0.12 Aa 1.44 ± 0.25 Ab 1.22 ± 0.23 Ab 0.71 ± 0.11 Ac 0.68 ± 0.18 Ac
    4(一次叠加) 1.02 ± 0.10 Aa 1.34 ± 0.20 Ab 1.19 ± 0.25 Ab 0.67 ± 0.18 Ac 0.61 ± 0.21 Ac
    12(一次污染) 3.65 ± 0.21 Ba 4.85 ± 0.23 Bb 4.38 ± 0.25 Bb 4.05 ± 0.30 Bc 3.73 ± 0.28 Bc
    12(三次叠加) 3.85 ± 0.18 Ba 4.15 ± 0.20 Ca 4.81 ± 0.25 Cb 5.02 ± 0.27 Cb 5.21 ± 0.26 Cb
    20(一次污染) 6.65 ± 0.22 Ca 9.66 ± 0.26 Db 9.45 ± 0.31 Db 9.13 ± 0.26 Db 8.84 ± 0.17 Db
    20(五次叠加) 6.38 ± 0.18 Ca 8.60 ± 0.18 Eb 10.05 ± 0.26 Ec 10.14 ± 0.31 Ed 11.04 ± 0.34 Ee
      注:同一行数据不同小写字母表示同一污染条件、相同浓度处理组,不同暴露时间差异之间蚯蚓体内毒死蜱蓄积量差异显著(P < 0.05);同一列数据不同大写字母表示同一污染条件、相同暴露时间,不同污染浓度处理组间蚯蚓体内毒死蜱蓄积量差异显著(P < 0.05)。
    下载: 导出CSV

    表  2  土壤生物蚯蚓-土壤毒死蜱蓄积因子

    Table  2.   Chlorpyrifos biota-soil accumulation factor of the earthworm in soil exposed to chlorpyrifos during the experimental period

    处理组(mg kg−1
    Treatment
    蓄积因子(FBSA
    Chlorpyrifos biota-soil accumulation factor of the earthworm
    21 d28 d35 d45 d55 d
    4(一次污染) 0.77 1.26 1.22 1.2 1.02
    4(一次叠加) 0.81 1.13 1.2 1.04 1.06
    12(一次污染) 0.91 1.33 1.33 1.38 1.55
    12(三次叠加) 1.11 1.35 1.67 2.01 2.22
    20(一次污染) 0.93 1.47 1.55 1.57 1.7
    20(五次叠加) 1.33 1.35 1.78 2 2.46
    下载: 导出CSV

    表  3  不同污染方式进入土壤的毒死蜱对蚯蚓的生长影响

    Table  3.   Effect of different chlorpyrifos application on growth of earthworms

    处理组(mg kg−1
    Treatment
    蚯蚓平均重量(mg)
    Mean weight per earthworm
    21 d28 d35 d45 d55 d
    对照组 508.5 ± 18.1 aA 512.5 ± 19.5 aA 518.4 ± 16.2 aA 520.5 ± 19.3 aA 522.3 ± 17.6 aA
    4(一次污染) 505.1 ± 16.5 aA 498.2 ± 18.8 aA 491.7 ± 19.3 aA 508.5 ± 10.2 aA 511.4 ± 10.2 aA
    4(一次叠加) 495.3 ± 17.2 aA 502.8 ± 19.4 aA 505.4 ± 10.3 aA 511.1 ± 11.2 aA 515.7 ± 9.8 aA
    12(一次污染) 485.7 ± 6.5 aA 481.8 ± 20.3 aA 457.8 ± 12.5 bB 451.3 ± 16.1 bB 457.2 ± 16.2 bB
    12(三次叠加) 495.2 ± 10.1 aA 485.7 ± 12.2 aA 465.5 ± 14.1 bB 421.6 ± 15.7 bC 404.2 ± 10.6 bC
    20(一次污染) 470.8 ± 12.3 aA 477.2 ± 20.6 aA 460.7 ± 14.4 bB 452.8 ± 13.3 bB 450.2 ± 20.3 bB
    20(五次叠加) 482.6 ± 19.6 aA 491.9 ± 21.1 aA 455.6 ± 18.8 bB 414.6 ± 20.3 cC 361.6 ± 22.1 cD
      注:同一行数据不同小写字母表示同一污染条件、相同浓度处理组,不同暴露时间之间蚯蚓体内毒死蜱蓄积量差异显著(P < 0.05);同一列数据不同大写字母表示同一污染条件、相同暴露时间,不同污染浓度处理组间蚯蚓体内毒死蜱蓄积量差异显著(P < 0.05)。
    下载: 导出CSV
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  • 收稿日期:  2020-07-14
  • 修回日期:  2020-11-02
  • 刊出日期:  2021-08-06

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