Abstract: Taking advantage of the opportunity of collecting a large number of samples from the Guizhou Province Cultivated Land Quality Geochemical Survey System, conduct research on the distribution of selenium (Se) in cultivated land soil and useful organs of crops. Statistical analysis showed that most of the surface soils in Zunyi was at full or rich levels of Se, only a very small proportion of surface soils was at deficient or high levels of Se. The content of Se in the soil is significantly correlated with the content of soil organic matter and altitude. It is obviously controlled by the lithology of the soil-forming parent rock. The correlation is not obvious when the soil pH value is < 8.5, and there is a significant negative correlation when the pH value is > 8.5. The Se content was decreased vertically with soil depths, which may be controlled by the content of soil organic matter in each layer. The Se content in crop depended mainly on the organ and growth period of crop. The Se content in corn and tea may be affected by the Se content in root soil.