Abstract:
Objective To provide the conversion and utilization of soil phosphorus (P) and to form a certain scientific theory basis in subtropical areas, the effects of P-solubilizing bacteria (PSB) on P fractions and bacterial diversities and community structures in soil were studied.
Method Experiment was carried out when the suspension of Burkholderia ZP-4 and Klebsiella ZP-2 was inoculated. Soil bacterial OTUs was measured using the high-through sequencing, and then soil bacterial diversities and soil properties were analyzed at treatments of B. ZP-4 and K. ZP-2.
Result The soil pH value and soil organic matter content were decreased significantly at treatments of B. ZP-4 and K. ZP-2, but soil available potassium, available nitrogen concentrations had significant increased relative to control. Contents of H2O-Pi, NaHCO3-Pi, NaHCO3-Po, NaOH-Pi, NaOH-Po were increased but HCl-Pi and Residual-P concentrations were significantly decreased. Soil bacterial Simpson and Shannon diversity indices, Chao1and ACE richness estimators were all no significant differences. The relative abundances of Olsenella, Fusobacterium, Streptococcus, Serratia were significantly increased than that of control at genus level.
Conclusion The B. ZP-4 and K. ZP-2 were significantly decreased soil pH value, enhanced soil available nutrient, accelerated soil P element transformation, altered significantly soil bacterial community diversity. Hence, the B. ZP-4 and K. ZP-2 improved soil available nutrient contents and pH, inducted soil bacteria to alter soil P fraction, accelerate soil P cycling.
下载: