A meta-analysis of the responses of soil microbial residues to exogenous nitrogen addition
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摘要: 微生物残体是土壤有机碳库的重要贡献者。为明确外源氮添加对土壤微生物残体积累动态的影响,本文收集整理了1980—2020年已发表的文献,共选取122组试验观测数据,利用整合分析方法(Meta-analysis),以微生物残体标识物-氨基糖为目标组分,定量分析了不同种类和数量的外源氮添加对土壤中微生物来源细胞残体积累数量和组成比例的影响,并系统解析其主要影响因素。结果表明:外源氮添加(0 ~ 6000 kg hm−1)对微生物细胞残体的积累有显著的促进作用,并能引起土壤中真菌和细菌来源细胞残体相对比例发生明显变化。与不加氮对照相比,氮添加使土壤氨基糖总量增加27%,其中氨基葡萄糖、氨基半乳糖和胞壁酸含量分别增加22.5%、29.8%和19.0%。同时,不同种类外源氮素添加对氨基糖积累特征的影响也有所不同,表现为有机氮(如动物厩肥)比无机氮添加对氨基糖积累的促进作用更大。此外,氮添加对氨基糖的影响程度还与土壤自身的碳氮比、土地利用类型和自然降雨量等环境因子密切相关。其中是否添加碳源对微生物残体的响应有较大影响,表现为:无碳源添加会降低土壤氨基糖葡萄糖和胞壁酸对氮添加的响应,削弱了微生物残体对土壤有机质的贡献比例;而氮源同时配合碳源添加条件下,土壤氨基糖积累量显著高于单一氮源添加的处理,说明氮添加对微生物残体积累的影响存在着碳氮耦合效应。Abstract: Microbial residue is an important contributor to soil organic carbon pool. In order to clarify the effects of exogenous nitrogen (N) addition on the accumulation dynamics of soil microbial residues, 122 groups of experimental observation data were collected from literatures published from 1980 to 2020. Meta-analysis method was used to analyze the effects of exogenous N addition on the accumulation dynamics of soil microbial residues, with amino sugars as the target component of microbial residues. The effects of quantity and type of N addition on the accumulation quantity and composition ratio of residues from different microbial sources in soil were analyzed quantitatively, and the main influencing factors were analyzed systematically. The results of integrated analysis showed that the addition of exogenous N (0 ~ 6000 kg hm−1) significantly promoted the accumulation of microbial residues, and caused a significant change in the relative proportion of fungal and bacterial residues in the soil. Compared with the control without N source, the contents of total amino sugars in soil increased by 27%, among which the contents of glucosamine, galactosamine and muramic acid increased by 22.5%, 29.8% and 19.0%, respectively. The effects of different kinds of exogenous N on the accumulation characteristics of amino sugar were also different, which showed that the addition of organic N (such as animal manure) promoted the accumulation of amino sugars more than that of inorganic N. In addition, the effect of N addition on amino sugar was also closely related to environmental factors such as soil C/N ratio, land use type and natural rainfall. Whether carbon source is added or not had a great impact on the response of microbial residues, which was shown as follows: the addition of carbon source did not reduce the response of soil glucosamine and muramic acid to N addition, and weakened the contribution proportion of microbial residues to soil organic matter; Under the condition of N source combined with carbon source, the accumulation of soil amino sugar was significantly higher than that of single N source. This indicates that there is a carbon-nitrogen coupling effect on the accumulation of microbial residues.
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Key words:
- Microbial residue /
- Amino sugar /
- Nitrogen addition /
- Meta-analysis
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表 1 数据分析所用文献
Table 1. The list of references associated with data analysis
参考文献
Reference地理位置
Geographic location年均温度(℃)
MAT年均降水量(mm)
MAP培养时间
Duration氮素类型
Nitrogen type土壤类型
Soil type丁雪丽等,2011[8] 41°49′N,123°34′E − − 266 d 有机无机氮混合
(NH4+ + 秸秆)淋溶土 丁雪丽等,2009[9] 43°36′N,124°40′E − − 266 d 有机无机氮混合
(NH4+ + 秸秆)软土 何红波等,2010[10] 43°34′N,124°38′E − − 126 d 无机氮
(NH4+)软土 He et al,2011[11] 43°30′N,124°48′E − − 147 d 无机氮
(NH4+)软土 He et al,2011[12] 43°31′N,124°49′E − − 147 d 无机氮
(NH4+)− 李晓波等,2011[7] 43°36′N,124°40′E − − 42 d 无机氮
(Urea)软土 Liang et al,2007[13] 28°46′N,115°50′E − − 210 d 无机氮
(NH4+)淋溶土 Murugan et al,2013[14] 54°27′N,9°57′E 8.9 768 90 d 有机氮
(牛浆)− Chen et al,2020[15] 39°55′N,115°20′E 5.4 500 6 a 无机氮 淋溶土 Chen et al,2020[16] 29°15′N,111°31′E − − 6 a 无机氮
(NH4+)− Ding et al,2015[17] 47°26′N,126°38′E 1.5 550 11 a 有机无机氮混合
(Urea + NH4+ + 粪肥)软土 Ding et al,2013[18] 47°26′N,126°38′E 1.5 550 10 a 有机无机氮混合
(Urea + NH4+ + 粪肥)软土 Ding et al,2013[19] 47°26′N,126°38′E 1.5 550 18 a 有机无机氮混合
(Urea + NH4+ + 粪肥)软土 Ding et al,2013[6] 47°26′N,126°39′E 1.5 550 21 a 有机无机氮混合
(Urea + NH4+ + 粪肥)软土 Fan et al,2020[4] 26°11′N,117°28′E 19.4 1700 5.5 a 无机氮
(NH4NO3)氧化土 Liu et al,2019[20] 41°31′N,123°24′E 7.5 700 1 a 有机无机氮混合
(NH4+ + 秸秆)淋溶土 Liang et al,2013[21] southern Wisconsin,USA − − 13.8 a 无机氮 − 47°10′N,90°50′E Ma et al,2020[22] 18°43′N,108°53′E 19.7 2198 7 a 无机氮
(NH4NO3)始成土 Peltre et al,2017[23] 55°40′N,12°16′E − − 12 a 有机氮
(粪肥)淋溶土 Zhang et al,2016[24] 23°10′N,112°10′E − − 11 a 无机氮
(NH4NO3)氧化土 -
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