接种根瘤菌对重茬豌豆土壤养分及酶活性的影响

张小红; 马绍英; 李胜; 曾芳荣; 马蕾; 杨宁; 蒲志辉

doi:10.19336/j.cnki.trtb.2021102106

接种根瘤菌对重茬豌豆土壤养分及酶活性的影响

Effects of Rhizobium Inoculation on Soil Nutrients and Enzyme Activities of Continuous Crop Pea

摘要

摘要:
目的探索解决豌豆重茬导致减产的问题。
方法以陇豌6号、定豌8号为对象，采用盆栽的方式探究外源接种单一、复合根瘤菌对重茬豌豆土壤养分及酶活性的影响。
结果 ①重茬种植降低了陇豌6号、定豌8号土壤养分含量及酶活性；外源接种根瘤菌可增加土壤硝态氮、铵态氮、有效磷、速效钾含量，提高土壤蔗糖酶、脲酶、碱性磷酸酶活性。②单一菌种、复合菌种的接种效果因不同生育时期及不同指标存在差异。③隶属函数综合分析表明，陇豌6号接种单一菌种‘ACCC15657’处理的平均隶属函数值最大（0.593），对土壤缓解效应最佳。定豌8号接种复合菌种‘ACCC15657 + ACCC15735 + Da99’处理的平均隶属函数值最大（0.533），对土壤缓解效应最强。
结论接种适宜根瘤菌可改善重茬土壤养分及酶活性，缓解豌豆连作障碍。

Abstract:
Objective The problems of legumes in continuous cropping are needed to be explored.
Method The effects of exogenous inoculation of single and compound Rhizobia on soil nutrients and enzyme activities of continuous pea were investigated using ‘Longwan 6’ and ‘Dingwan 8’ as objects.
Result ① Continuous cropping decreased the soil nutrient contents and enzyme activities of ‘Longwan 6’ and ‘Dingwan 8’. Exogenous rhizobium inoculation could boost soil nitrate nitrogen, ammonium nitrogen, available phosphorus, and available potassium levels, as well as soil activities of sucrase, urease alkaline phosphatase. ② The inoculation effect of single strain and compound strain was different due to different growth stages and different indices. ③ The average membership function value of ‘Longwan 6’ inoculated with a single strain ‘Accc15657’ was the largest (0.593), and the mitigating impact on the soil was the best. Ding wan 8 inoculated with compound strain ‘Accc15657 + Accc15735 + Da99’ had the highest average membership function value (0.533), and had the strongest mitigation effect on soil.
Conclusion Inoculating suitable rhizobia can boost soil nutrients and enzyme activities during continuous cropping, as well as reduce the pea continuous cropping issue.

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